|Superclasses:||a redox electron carrier|
Studies in the 1950's have shown that glyceraldehyde 3-phosphate dehydrogenase slowly catalyzes the formation of NADHX from NADH [Rafter54]. The equilibrium of this hydration reaction is in favor (100/1) of the hydrated form [Acheson88], making the reaction virtually irreversible.
NADHX and NADPHX are inhibitors of several dehydrogenases [Prabhakar98] and it is thus important to eliminate them. Reconversion of NADHX to NADH is catalyzed by an ATP-dependent dehydratase [Yoshida75, Marbaix11].
Chemical Formula: C21H29N7O15P2
Molecular Weight: 681.44 Daltons
Monoisotopic Molecular Weight: 683.1353363829999 Daltons
Marbaix11: Marbaix AY, Noel G, Detroux AM, Vertommen D, Van Schaftingen E, Linster CL (2011). "Extremely Conserved ATP- or ADP-dependent Enzymatic System for Nicotinamide Nucleotide Repair." J Biol Chem 286(48);41246-52. PMID: 21994945
Prabhakar98: Prabhakar P, Laboy JI, Wang J, Budker T, Din ZZ, Chobanian M, Fahien LA (1998). "Effect of NADH-X on cytosolic glycerol-3-phosphate dehydrogenase." Arch Biochem Biophys 360(2);195-205. PMID: 9851831
Rafter54: Rafter, G. W., Chaykin, S., Krebs, E. G. (1954). "The action of glyceraldehyde-3-phosphate dehydrogenase on reduced diphosphopyridine nucleotide." J. Biol. Chem. 208: 799-812. PMID: 13174589
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