Definition: The process of coupling proline to prolyl-tRNA, catalyzed by prolyl-tRNA synthetase. In tRNA aminoacylation, the amino acid is first activated by linkage to AMP and then transferred to either the 2'- or the 3'-hydroxyl group of the 3'-adenosine residue of the tRNA.
GO:0006418 - tRNA aminoacylation for protein translation
prolyl-tRNA synthetase (proS)
Unification Links: GO:0006433
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